Reliable Desmin Antibody Vet Pathology

November 10, 2026

BlueskyReddit
Afyon Kocatepe University

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Company:

Agilent Technologies

Product Name:

Desmin

Catalog Number:

M0760

Clone Number:

D33

Info:Supplier Page

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I use desmin in our veterinary pathology laboratory to evaluate muscle differentiation in tumor samples. It is particularly helpful in suspected rhabdomyosarcomas and other mesenchymal tumors.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Dog muscle neoplasia

Primary Incubation

1/20 dilution / 2 hours at RT

Blocking Agent

BSA

Secondary Incubation

Biotinylated Anti-Mouse IgG - 1/200 dilution

Tertiary Incubation

ABC-HRP (1/50 dilution)

Detection

Avidin–Biotin Complex (ABC) Method

Results Summary

I used this antibody at a 1:20 dilution with a 2-hour incubation at room temperature. It worked very well in a canine tissues and showing clear cytoplasmic staining. The staining helped us evaluate muscle differentiation and supported our diagnosis.

Additional Notes

AEC choromagen / Gill's III hematoxylin

Image Gallery

Summary

The Good

Good cytoplasmic staining in canine tumor tissue. Easy to interpret and works well with the conventional ABC-HRP method.

The Bad

nope, maybe low dilution (1/20)

The Bottom Line

A very useful antibody for veterinary pathology. We obtained excellent results in a canine rhabdomyosarcoma. I would recommend this antibody for evaluating muscle differentiation in animal tumors.

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