Excellent and Affordable PAX5 Marker

November 07, 2026

BlueskyReddit
Afyon Kocatepe University

Overall

Quality of Results

Ease-of-Optimization

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Company:

Zeta Corporatin

Product Name:

PAX-5

Catalog Number:

Z2354ML

Clone Number:

ZM26

Info:Supplier Page

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This PAX5 antibody to identify B lymphocytes in canine and feline tissues, particularly in suspected lymphoma cases. It works very well in both species and has become a valuable marker in our routine diagnostic pathology work for vet pathology labs.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Cat skin (cutaneous lymphoma)

Primary Incubation

1/100 dilution / 2 hours at RT

Blocking Agent

BSA

Secondary Incubation

Biotinylated Anti-Mouse IgG - 1/200 dilution

Tertiary Incubation

ABC-HRP (1/50 dilution)

Detection

Avidin–Biotin Complex (ABC) Method

Results Summary

This antibody gave excellent results in both feline and canine tissues at a 1:100 dilution. The nuclear staining was strong, sharp, and easy to interpret. I was particularly impressed by its performance, especially considering its affordable price.

Additional Notes

DAB choromagen / Gill's III hematoxylin

Image Gallery

Summary

The Good

Excellent nuclear staining, works very well in cats and dogs, and is surprisingly affordable.

The Bad

No significant problems so far.

The Bottom Line

One of the best value-for-money PAX5 antibodies I have used. Excellent results in both cats and dogs at a 1:100 dilution. Highly recommended for veterinary pathology laboratories.

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