Biolegend
PE anti-human HLA-A,B,C Antibody
311405
W6/32
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We wanted to validate surface expression of HLA A,B,C in a human melanoma cell line. To test the antibody, we treated cells with and without IFN gamma before observing expression.
Flow Cytometry
A375 human melanoma cells
1:400, 30 min 4C
FACS buffer (PBS + 5% FBS + 0.05% sodium azide
N/A
Flow cytometry
We were able to successfully validate surface expression of HLA A,B,C on A375 cells. Expression increased with IFN gamma treatment, as expected.
Worked as expected
Worked well, will be using for future experiments to determine if drug treatment affects surface expression of HLA A, B, C.