Cell Signaling Technologies
Cleaved Caspase-3 (Asp175) Antibody
cst9661
Browse Similar Products: Cleaved-Caspase 3 Antibody Products
We work on using stem cells to model and treat rare pediatric diseases. We have utilized this antibody in a variety of cell types to stain for cleaved caspase 3 for qualitative and quantitative assessment of apoptotic cells.
Immunofluorescence
Human iPSCs
Diluted 1:400 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Rb. Cy3 IgG (Jackson Immunoresearch) 1:500 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Hoescht 1:10,000 in 1X PBS for 5mins at room temperature
Imaged on EVOS Fl Auto at 20x magnification
Multiple iPSCs stained positive for cleaved-caspase 3 marker indicating apoptotic potential. Assay has been used to quantify cell death.
doi: 10.1002/2211-5463.70136
Affordable and consistent staining
None
Great antibody for CC3 immunofluorescent staining across multiple cell types