Cell Signaling Technologies
HSP60 (D6F1) Rabbit Monoclonal Antibody
cst12165
D6F1
Browse Similar Products: HSP60 Antibodies
We work on using stem cells to model and treat rare pediatric diseases. We work on a rare metabolic disease caused by ETFA mutations and use this antibody on patient-derived cells for various assays to stain for mitochondrial structure and physiology.
Immunofluorescence
Human fibroblasts
Diluted 1:500 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Rb. Cy3 IgG (Jackson Immunoresearch) 1:500 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Hoescht 1:10,000 in 1X PBS for 5mins at room temperature
Imaged on EVOS Fl Auto at 40x magnification
Antibody stained human fibroblasts well with clear mitochondrial staining. Staining was confirmed by colocalization with another mitochondrial marker, ETFA.
Affordable with clear staining
Slightly dim so could increase concentration
Overall, affordable and consistent antibody for mitochondrial staining but could be better optimized