Cell Signaling Technologies
Oct-4 Antibody
cst2750
Browse Similar Products: Oct-4 Antibody Products
We work on using stem cells to model and treat rare pediatric diseases. We reprogram patient-derived fibroblasts into iPSCs and utilize this antibody for validation of pluripotency.
Immunofluorescence
Human iPSCs
Diluted 1:200 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Rb. Cy3 IgG (Jackson Immunoresearch) 1:500 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Hoescht 1:10,000 in 1X PBS for 5mins at room temperature
Imaged on EVOS Fl Auto at 20x magnification
The antibody positively stained the imaged reprogrammed iPSC cluster indicating successful reprogramming and pluripotency. Staining was slightly dim which could be optimized with higher antibody concentration. Imaging was further validated by staining with other pluripotency markers.
doi: 10.1002/2211-5463.70136
Affordable with clear cell-specific staining
Dim when imaged
Overall, a standard Oct4 antibody that demonstrates cell-specific staining for pluripotency validation but is slightly dim on imaging with room for optimization.