Invitrogen
CytoTune‐iPS 2.0 Reprogramming Kit
A16517
Info:Supplier Page
I work on using stem cell models to understand and treat rare pediatric diseases. This product has been utilized in our lab to reprogram patient-derived fibroblasts into iPSCs which are used for further analysis and differentiation
iPSC Viral Reprogramming
Human-derived fibroblasts
Kit can be used for multiple reprogramming. Ensure MOIs are calculated accurately and kit is stored in -80C after opening.
The kit has successfully reprogrammed various patient lines into iPSCs (confirmed with pluripotency staining and embryoid body assay). Multiple clones are produced per line. Image demonstrates reprogramming timeline.
https://pmc.ncbi.nlm.nih.gov/articles/PMC12871563/#feb470136-sec-0002
Easy reprogramming steps, great efficiency
Process takes ~30days and requires high-level of planning and calculations
Overall, a great viral kit to reprogram fibroblasts into iPSCs with good efficiency