Thermo Fisher Scientific
GRP78 Polyclonal Antibody
PA1-014A
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I work on using stem cell models to understand and treat rare pediatric diseases. This product works well for western blotting and has been used across a multitude of human patient-derived cell types including fibroblasts, iPSCs, and differentiated cells. It forms clean bands even at lower dilutions.
Western Blot
Human iPSCs
Diluted 1:1000 in TBST+5% BSA for 1hr at room temperature
TBST+5% BSA
Rb.800 IRDye diluted 1:10,000 in TBST+5% BSA for 1hr at room temperature
None
Imaged on Licor Odyssey FX
The antibody produced clean bands at 78kDa and demonstrated no change across studied genotypes of disease and control patients. It was easily quantified with clean banding and good signal.
Clear banding at low dilution
Moderate cost
Overall, a solid antibody for GRP78/BiP western blotting