A Reliable Nuclear B-Cell Marker for Dogs and Cats

November 03, 2026

BlueskyReddit
Afyon Kocatepe University

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Company:

Agilent

Product Name:

Pax5

Catalog Number:

M7307

Clone Number:

Dak-Pax5

Info:Supplier Page

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We routinely use PAX5 to identify B lymphocytes in dog and cat samples, especially in suspected lymphoma cases. It gives clear nuclear staining and has been very helpful in our daily diagnostic work. In my experience, it is one of the most useful markers in our immunohistochemistry panel.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Cat intestinal lymphoma

Primary Incubation

1/50 dilution / 2 hours at RT

Blocking Agent

BSA

Secondary Incubation

Biotinylated Anti-Mouse IgG - 1/200 dilution

Tertiary Incubation

ABC-HRP (1/50 dilution)

Detection

Avidin–Biotin Complex (ABC) Method

Results Summary

We used this antibody with the ABC-HRP immunohistochemistry method and antigen retrieval at pH 6.0. It produced excellent, strong, and clear nuclear staining in feline lymphoma samples, with minimal background staining. The results were consistent and easy to interpret, making this antibody very useful in our routine diagnostic work.

Additional Notes

DAB choromagen / Gill's III hematoxylin

Image Gallery

Summary

The Good

Very sharp staining in both feline and canine tissues.

The Bad

nope, maybe low dilution (1/50)

The Bottom Line

Excellent antibody for both cat and dog tissues. Strong, sharp nuclear staining and very reliable results. I would definitely recommend it.

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