Abcam
Rat Anti-Myelin Basic Protein (MBP) antibody
ab7349
12
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We work on using stem cells to model and treat rare pediatric diseases, especially demyelinating diseases. We differentiate patient-derived iPSCs into neural cells including oligodendrocytes and utilize this antibody as a myelin immunofluorescence marker.
Immunofluorescence
Human differentiated oligodendrocytes
Diluted 1:1000 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Rt. Cy5 IgG (Jackson Immunoresearch) 1:500 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Hoescht 1:10,000 in 1X PBS for 5mins at room temperature
Imaged on Nikon Spinning Disk at 100x magnification
Patient-derived iPSCs from an ultra-rare demyelinating condition were differentiated into oligodendrocytes. Immunofluorescence with this antibody was used to stain myelin to quantify myelin quantity as well as observe abnormal myelin structure.
https://doi.org/10.1186/s12967-026-08708-8
Clear specific staining, low concentration required
Higher cost
Overall, a great myelin antibody with low concentration required and consistent staining. The rat host allows for co-staining with various other antibodies.