Cell Signaling Technologies
Beta-III-Tubulin (TU-20) Mouse Monoclonal Antibody
cst4466S
TU20
Browse Similar Products: Beta-III-Tubulin Antibody Products
We work on using stem cells to model and treat rare pediatric diseases. We differentiate patient-derived iPSCs into neuronal cells and utilize this antibody as a neural progenitor and neuron marker
Immunofluorescence
Human differentiated neurons
Diluted 1:200 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Rb. Cy3 IgG (Jackson Immunoresearch) 1:500 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Hoescht 1:10,000 in 1X PBS for 5mins at room temperature
Imaged on EVOS Fl Auto at 40x magnification
Patient-derived iPSCs from rare neurological conditions were differentiated into neurons utilizing a dual SMAD inhibition protocol. Immunofluorescence with this antibody has stained neurons confirming cell identity. The antibody is also used for structural analysis and neuronal identification in mixed cultures.
https://www.cellsignal.com/products/primary-antibodies/map2-antibody/4542
Clear specific staining
None
A great and consistent marker for neurons