Phosphatase Inhibitor Cocktail

September 28, 2026

BlueskyReddit
Stanford University
Biochemistry
Senior Research Scientist

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Company:

Roche

Product Name:

PhosSTOP

Catalog Number:

4906837001

WNT signaling is essential for embryonic development and adult tissue homeostasis, and its aberrant activation drives several cancers, most notably colorectal cancer. My lab studies the molecular mechanisms of oncogenic WNT signal transduction, with the goal of identifying therapeutic vulnerabilities in colorectal cancer. Because β-catenin turnover by the destruction complex depends on its phosphorylation, preserving phosphorylation during cell lysis is essential for our experiments. I use Roche PhosSTOP™ EASYpack phosphatase inhibitor tablets (Cat#4906837001) in mammalian cell lysis buffers.

Experimental Design and Results Summary

Application

Protein Biochemistry

Starting Material

Mammalian cells

Tips

None

Results Summary

I use PhosSTOP for phosphatase inhibition in experiments where I add a protease inhibitor cocktail separately. This lets me control the two inhibitor types independently, depending on the needs of the experiment. The tablets protect phosphorylated proteins in our lysates, including β-catenin, whose phosphorylation state is a central readout in our destruction complex studies.

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Summary

The Good

PhosSTOP is a dedicated phosphatase inhibitor, so it pairs flexibly with whichever protease inhibitor cocktail an experiment calls for. Each tablet is pre-measured for 10 mL of buffer, so there's no weighing or preparation of individual inhibitors. Its stated coverage includes the serine/threonine phosphatases PP1, PP2A, and PP2B, which is particularly relevant for β-catenin, since PP2A is a component of the destruction complex.

The Bad

None

The Bottom Line

PhosSTOP is an effective and convenient phosphatase inhibitor for phosphorylation-sensitive work in mammalian cell lysates, and it is well suited to experiments where protease inhibitors are added separately. It is somewhat expensive, but for experiments where preserving phosphorylation is critical, it is a worthwhile choice.

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