Fast Cell Viability Assay

September 21, 2026

BlueskyReddit
Stanford University
Department of Pathology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review
Questions/Comments

Company:

Promega

Product Name:

CellTiter-Glo® 2.0 Cell Viability Assay

Catalog Number:

G9242

I study gene signaling pathways and routinely measure cell viability and responses to experimental treatments. CellTiter-Glo 2.0 provides a simple luminescence-based readout of ATP and is convenient for multiwell experiments

Experimental Design and Results Summary

Application

Cell viability and proliferation assays

Starting Material

Cultured human cells

Tips

Avoid using edge wells as they have edge-effect on cell growth

Results Summary

The assay uses an add-and-read format and works well for plate-based experiments and higher-throughput studies. I used it to quantify cell viability based on ATP levels and compare cell responses across experimental conditions within 15 min.

Image Gallery

Summary

The Good

Fast, simple, sensitive, and easy to scale to multiwell experiments.

The Bad

Requires a luminescence plate reader, expensive

The Bottom Line

A very convenient assay for quickly measuring cell viability and treatment responses without complicated processing.

Join the discussion