Efficient Myelin Isolation Beads

September 03, 2026

BlueskyReddit
State University of New York at Buffalo
Physiology and Biophysics
Postdoctoral Researcher

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Quality of Results

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Questions/Comments

Company:

Miltenyi Biotec

Product Name:

Myelin Isolation Beads, human, mouse, rat

Catalog Number:

130-104-262

We routinely isolate astrocytes from mouse brain tissue in our lab for different downstream applications (Seahorse, Western blot, PCR, RNA-seq, etc.). Myelin isolation is an important step for achieving high-quality astrocyte isolation, ensuring high purity and yield, especially when working with adult mice (we work with mice older than postnatal day 60).

Experimental Design and Results Summary

Application

Magnetic sorting (cell isolation)

Starting Material

Fresh mouse brain tissue (hippocampus, striatum, etc.)

Tips

We work with small tissue samples and add 15 µL of myelin isolation beads to the pellet together with 135 µL of PBS/BSA buffer. The samples are then incubated for 15 minutes before magnetic separation.

Results Summary

We were able to isolate high-purity astrocytes using both magnetic sorting and FACS when myelin was removed beforehand. This allowed us to proceed with the isolated astrocytes for various downstream applications, including RNA-seq, which confirmed the high quality of the isolated material.

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Summary

The Good

Reliable myelin removal and an easy-to-use reagent.

The Bad

The shelf life is short, so it should be ordered prior to the experiments.

The Bottom Line

Worked very well throughout the experiments, allowing us to isolate pure cells with high yield. Highly recommended.

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