CellMask™ Deep Red Staining in HBMECs Requires Optimization

September 02, 2026

BlueskyReddit
Cell & Tissue Engineering, Imec
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Invitrogen

Product Name:

CellMask™ Deep Red Plasma Membrane Stain

Catalog Number:

C10046

Info:Supplier Page

Browse Similar Products: Plasma membrane stain kits

CellMask Deep Red was used to stain the plasma membrane of live primary human brain microvascular endothelial cells (HBMECs).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

human brain microvascular endothelial cells

Primary Incubation

Different concentrations of working solution were tested (0.5X–1.5X), in HBSS.

Blocking Agent

none

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fluorescent microscopy

Results Summary

We observed a short period of efficient staining with HBMECs using this CellMask. Different working concentrations (0.5×, 1×, and 1.5×) were tested. Among these, the lowest concentration (0.5×) resulted in the least cell death. The internalization was fast (~10 min).

Additional Notes

Prepare a fresh working solution

Image Gallery

Summary

The Good

Fast method for detection of the plasma membrane in live cells

The Bad

Requires optimization in HBMECs to avoid causing cell death

The Bottom Line

These observations were assessed only in HBMECs, which might be different for other cell types. Nevertheless, it is a fast staining method, at a reasonable price.

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