2X PCR Master Mix

August 28, 2026

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Uka Tarsadia University

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific

Product Name:

PCR Master Mix (2X) Thermo Scientific

Catalog Number:

K0171

The aim of the experiment was to genotype HLAB27 by PCR SSP method. Genomic DNA was subjected to PCR using 2x PCR Master Mix.

Experimental Design and Results Summary

Application

Polymerase Chain Reaction

Starting Material

Genomic DNA

Tips

Good quality and optimum quality yield good specific amplified product.

Results Summary

The master mix resulted in good amplified product. It is useful in methods like PCR SSP and no need of much optimisation.

DOI or PMID #

https://doi.org/10.1080/15257770.2024.2303710

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Summary

The Good

It contains optimum concentration of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers

The Bad

Good quality of DNA is needed

The Bottom Line

The 2X PCR master mix (Thermo Scientific, USA) is very well optimised for PCR amplification. This pack size can be used for 200 reactions.

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