Great SYBR Green

UNC Chapel Hill

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

PowerUp™ SYBR™ Green Master Mix

Catalog Number:

A25742

Applied Biosystems SYBR Green Master Mix consistently delivers robust qPCR performance with minimal optimization. Amplification was efficient, melt curves were clean, and the master mix generated highly reproducible Ct values across technical replicates. It is compatible with a wide variety of primer sets and provides dependable performance for routine gene expression studies.

Experimental Design and Results Summary

Application

qpcr

Starting Material

Cells

Tips

Keep in appropriate temp

Results Summary

The master mix produced strong amplification signals with clean, single-peak melt curves and little evidence of nonspecific amplification. Ct values were highly reproducible across replicates, while amplification efficiencies remained consistent between runs. The results supported accurate and reliable quantitative gene expression analysis.

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Summary

The Good

easy to use

The Bad

expensive

The Bottom Line

using it for years. it is great

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