Recombinant IL-2 Protein, Human for T Cell Stimulation

August 18, 2026

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Uka Tarsadia University

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Company:

Thermo Fisher Scientific

Product Name:

Human IL-2 Recombinant Protein, PeproTech®

Catalog Number:

200-02-1MG

The aim of the experiment was to stimulate T cells in a co-culture system of different subsets of T cells isolated from whole blood of humans. The goal of the experiment was to carry out cell suppression assay after T cell stimulation.

Experimental Design and Results Summary

Application

Cell culture, Cell stimulation, Cell expansion

Starting Material

T cells (Human)

Tips

Storage conditions -20°C

Results Summary

We used rIL-2 (200 IU) (PeproTech) and anti-CD3/anti-CD28 dynabeads at 1:1 bead to cell ratio for 5 days at 370C and 5% CO2 in 96 well U bottom plate. In our experiments we successfully stimulate T-cell activation and proliferation in cell culture.

DOI or PMID #

doi: 10.1016/j.intimp.2023.111174

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Summary

The Good

Contain sufficient quantity and reliable for T cell stimulation. It does not contain preservative.

The Bad

Optimization is needed for the optimal concentration for cell stimulation which is based on the specific cell type being used (e.g., human T cells, mouse splenocytes) and the goals of the experiment.

The Bottom Line

rIL-2 (200 IU) (PeproTech) is very apt to stimulate T cells in cell culture system. For short-term activation (e.g., 24–48 hours) this cytokine can be used in combination with anti-CD3/CD28 antibodies to induce T-cell responses. For 48-hour T cell activation culture, 100 IU/mL IL-2 concentration can be used. However, for long-term culture and higher number of cells increase IL-2 dose to 200 U/mL from day 6 onward.

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