The Good
It is ready-to-use, reliable and efficient reagent for RNA isolation. It (Trizol ) can be useful for isolation of DNA and protein from it's organic phase.
The Bad
Optimization is needed due to different cell types of the experiments.
The Bottom Line
TRIzol Reagent (Invitrogen, Carlsbad, CA) is very apt to isolate total RNA from tissues or cells, including lipid-rich and difficult samples. Moreover, Trizol can also be used to isolate DNA and protein from it's organic phase. The protocol is very simple and results are consistent. However, little optimization (for Trizol volume to be taken) may be needed due to different cell and tissue types of the experiments.