Good Antibody for Detecting EVs

Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

CD9 Rabbit Monoclonal Antibody

Catalog Number:

#13403

Clone Number:

D3H4P

CD9 antibody was used to detect the extracellular vesicle(EVs). The results showed CD9 is clearly detected in isolated EV, indicating CD9 is a good EVs marker.

Experimental Design and Results Summary

Applications

Western Blot

Sample

extracellular vesicle

Primary Incubation

1:1000 overnight at 4 degree

Blocking Agent

5% milk in TBS

Secondary Incubation

1 hour at room temperature

Tertiary Incubation

n/a

Detection

ECL

Results Summary

Extracellular vesicle was isolated from cultured 293T media. CD9 antibody was used to detect the extracellular vesicle(EVs). The results showed CD9 is clearly detected in isolated EV, indicating CD9 is a good EVs marker.

Image Gallery

Summary

The Good

Antibody is good for EVs

The Bad

n/a

The Bottom Line

This antibody has good signaling in EVs sample.

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