Human Whole Blood Treg Isolation Kit

C G Bhakta Institute of Biotechnology
Associate Professor

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Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

MACSxpress® Whole Blood Treg Isolation Kit, human

Catalog Number:

130-109-557

The aim of the experiment was to isolate CD4+CD25+ regulatory T cells (Tregs) from whole blood of humans.

Experimental Design and Results Summary

Application

Isolation of Treg cells from human whole blood

Starting Material

Whole blood (human)

Tips

Additional Treg cell marker can be added (e.g., FOXP3)

Results Summary

The kits' efficacy and Treg-cell purity was confirmed by flow cytometry. The isolated CD4+ CD25+ Treg cells showed 84.01% purity.

DOI or PMID #

https://doi.org/10.1111/exd.14157

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Summary

The Good

The Treg isolation kit has well optimized protocol with very less time.

The Bad

The kit can include additional Treg cell marker such as FOXP3.

The Bottom Line

The Treg isolation kit from Miltenyi Biotec is very effcient to get adequate amount of Treg cells from human blood. However, the kit can include few additional Treg cell marker like FOXP3.

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