dsDNA Quantification Kit for Residual DNA Analysis After IVT mRNA Production

August 06, 2026

BlueskyReddit
Houston Methodist Research Institute

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Questions/Comments

Company:

Invitrogen

Product Name:

Quant-iT™ PicoGreen™ dsDNA Assay Kit

Catalog Number:

P11496

I used the Quant-iT™ PicoGreen™ dsDNA Assay Kit to quantify residual double-stranded DNA following in vitro transcription (IVT) mRNA production. The assay was straightforward to perform, highly reproducible, and provided accurate quantification using the standard protocol

Experimental Design and Results Summary

Application

Residual dsDNA Quantification

Starting Material

Purified IVT mRNA samples

Tips

I initially prepared a low-range standard curve, but the residual dsDNA concentration in my samples exceeded that range. Using the higher standard curve provided accurate quantification. Protect the PicoGreen reagent from light, incubate the plate for 5 minutes covered with aluminum foil, and then measure fluorescence using a plate reader.

Results Summary

The kit was used to quantify residual dsDNA following IVT mRNA production. After switching to the higher standard curve, the assay accurately measured dsDNA levels with excellent reproducibility. The workflow was simple, requiring only a 5-minute incubation protected from light before reading on a fluorescence plate reader.

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Summary

The Good

Easy protocol, rapid 5-minute incubation, sensitive detection, excellent reproducibility, and reliable quantification over a broad concentration range.

The Bad

Samples with higher dsDNA content may require a higher standard curve or additional dilution to remain within the assay's linear range.

The Bottom Line

A highly sensitive and easy-to-use assay for residual dsDNA quantification after IVT mRNA production. It provides reliable, reproducible results with minimal hands-on time.

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