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APC/Fire™ 750 anti-human CD41/CD61 Antibody
359816
A2A9/6
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Detecting megakaryocytes by flow cytometry is never easy. These cells are usually very large and fragile. Therefore, we needed to give our assay every chance of labelling them. We chose this antibody because it has both CD41 and CD61 together. Also, it was on a fluorochrome that we were able to use in our multi-color panel. A full titration was performed before adding it to the panel. The result from the optimal volume is shown in the plot.
Flow Cytometry
Human blood, bone marrow aspirate
15 minutes at Room Temp
None
N/A
Red Laser (633 nm) and a filter around 774 nm
The results were good. We can see, even at 2.5uL per test (data shown in the image attached), that the antibody stained the cells of interest. There was a very good separation between the negatives and the positives (acceptable resolution). The background was very low.
good price, good resolution
tough to compensate
If you need a good platelets/megakaryocyte marker, this will work nicely. But beware of compensation issues.