Attune Flow Cytometer

VIB
Senior Research Associate Translational Research - VIB Discovery Sciences

Overall

Performance

Ease-of-Optimization

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Company:

ThermoFisher Scientific

Product Name:

Attune NxT Flow Cytometer

Catalog Number:

A24858

We use the Attune Flow Cytometer to detect the expression of target proteins on cells' membrane. And also to screen binders to these protein.

Experimental Design and Results Summary

Application

Flow Cytometry

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Summary

The Good

Easy to use; Software gives instructions on how to fix some errors and on important steps (list of steps for start-up, cleaning, shutdown,...); can read a 96-well plate in 1h.

The Bad

Software has bugs, example: when starting a new experiment the "IS" icon doesn't appear on the samples, we need to drag and drop from a previous experiment, which is annoying if we have different cells (and parameters) in the same plate. Error sometimes are not correct: recently we had a clog and the error didn't mention it, instead that the volume of the sample was not enough; or one of the bottles was not plugged in correctly and the error message mentioned a clog

The Bottom Line

The Attunne is easy to use and when it's working properly is perfect; however once the problems, start we try all the different things and can't fix it, and most of the times the service technique needs to come and we lose the experiment.

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