A Sensitive DNA Labeling Kit

UNIVERSITY OF ALABAMA AT BIRMINGHAM
Pharmacology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Questions/Comments

Company:

Thermo Fisher Scientific

Product Name:

BioPrime DNA Labeling System

Catalog Number:

18094011

The kit was purchased to label genomic DNA probes for detecting correct DNA fragment insertion. Following the manufacturer's protocol, high-quality labeled probes were successfully generated. These probes enabled the identification and confirmation of correct DNA fragment insertion into the genomic DNA by Southern blot analysis.

Experimental Design and Results Summary

Application

DNA Labeling and Southern blot

Starting Material

Salmon Sperm DNA

Tips

extend reaction time to 3 hours

Results Summary

High-quality labeled probes were successfully generated using salmon sperm DNA as a carrier, following the manufacturer's protocol. These probes enabled the identification and confirmation of correct DNA fragment insertion into genomic DNA by Southern blot analysis , providing a reliable non-radioactive alternative for nucleic acid detection.

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Summary

The Good

sensitive and easy to label

The Bad

n/a

The Bottom Line

The kit is good to prepare biotinylated probe and provides a reliable non-radioactive alternative.

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