Reliable One-Step RT-qPCR Detection of Low-Input RNA from Adult Mouse Astrocytes

July 29, 2026

BlueskyReddit
SUNY UB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

iTaq™ Universal SYBR® Green One-Step Kit

Catalog Number:

1725151

We used the iTaq™ Universal SYBR® Green One-Step Kit to quantify gene expression directly from RNA isolated from immunomagnetically purified adult mouse astrocytes. The kit generated reproducible amplification curves, clean melting profiles, and consistent detection of Npas3, Slc25a18, Slc25a22, and Gapdh from low-input RNA samples.

Experimental Design and Results Summary

Application

One-step RT-qPCR

Starting Material

Total RNA isolated from adult mouse astrocytes

Tips

The one-step workflow simplified sample handling and reduced pipetting steps while maintaining excellent reproducibility. Four technical replicates per sample produced highly consistent Ct values.

Results Summary

The kit provided sensitive and reproducible quantification of multiple target genes from purified astrocytes using only 5 ng RNA per reaction. Amplification specificity was confirmed by dissociation curve analysis, and the workflow supported robust comparative gene-expression analysis using the ΔΔCt method.

DOI or PMID #

10.1126/sciadv.adt2527

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Summary

The Good

Excellent sensitivity, reproducible amplification, robust performance with low-input RNA, and straightforward workflow.

The Bad

No limitations were observed in our experiments.

The Bottom Line

An excellent one-step RT-qPCR kit for sensitive gene-expression analysis from limited RNA samples.

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