Cell Signaling Technologies
GFAP Monoclonal Antibody
cst3670
GA5
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We work on using stem cells to model and treat rare pediatric diseases. We differentiate patient-derived iPSCs into neural cells including astrocytes and utilize this antibody as an astrocyte immunofluorescence marker.
Immunofluorescence
Human differentiated astrocytes
Diluted 1:200 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Mo. Cy5 IgG (Jackson Immunoresearch) 1:500 in IF buffer (1X PBS+ 5% Normal Donkey Serum+0.3% Triton X-100) for 1hr at room temperature
Hoescht 1:10,000 in 1X PBS for 5mins at room temperature
Imaged on EVOS Fl Auto at 20x magnification
Patient-derived iPSCs from a rare neurological condition were differentiated into neural cell types. Immunofluorescence with this antibody has stained astrocytes in the mixed cell population allowing for further identification and analysis.
Clear signal, well cited
None
Great standard antibody for astrocyte staining