Not Working Mitochondrial Marker Antibody

July 23, 2026

BlueskyReddit
Health Division of Gastroenterology
UC Davis

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech

Product Name:

VDAC1/2 Polyclonal antibody

Catalog Number:

10866-1-AP

Clone Number:

We are interested in understanding the function of a endososome-localized enzyme and use this anti-VDAC1/2 antibody as mitochondrial marker to validate its subcellular localization not to be in mitochondria. However this antibody recognizes multiple bands at the wrong size.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293T cells

Primary Incubation

1:1000 dilution overnight in the cold room

Blocking Agent

3% BSA in PBS

Secondary Incubation

1:10000 goat anti-rabbit

Tertiary Incubation

1hr incubation at room temp

Detection

LICOR fluorescence

Results Summary

We are interested in understanding the function of a endososome-localized enzyme and use this anti-VDAC1/2 antibody as mitochondrial marker to validate its subcellular localization not to be in mitochondria. However this antibody recognizes multiple bands at the wrong size.

Image Gallery

Summary

The Good

Cheap

The Bad

Not working

The Bottom Line

This is not a good antibody to be used as a mitochondrial marker.

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