Highly Efficient Isolation of Adult Mouse Astrocytes Using Anti-ACSA-2 MicroBeads

July 23, 2026

BlueskyReddit
SUNY UB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review
Questions/Comments

Company:

Miltenyi Biotec

Product Name:

Anti-ACSA-2 MicroBeads

Catalog Number:

130-097-678

We used Anti-ACSA-2 MicroBeads to immunomagnetically isolate astrocytes from adult mouse prefrontal cortex for downstream protein analyses. The isolated astrocyte fraction showed strong enrichment for the astrocytic marker S100ß with minimal neuronal (NeuN) and microglial (Iba1) contamination, demonstrating excellent isolation specificity. The purified cells were fully compatible with Western blot analysis of mitochondrial proteins and astrocyte metabolism.

Experimental Design and Results Summary

Application

Immunomagnetic isolation of astrocytes

Starting Material

Adult mouse prefrontal cortex

Tips

Fresh tissue processing and careful adherence to the manufacturer's protocol produced highly reproducible astrocyte enrichment with excellent purity.

Results Summary

The magnetic isolation consistently generated highly enriched astrocyte populations suitable for downstream Western blot analysis. Validation demonstrated strong enrichment of the astrocyte marker S100β with minimal neuronal and microglial contamination, confirming excellent isolation specificity.

DOI or PMID #

10.1126/sciadv.adt2527

Image Gallery

Summary

The Good

Excellent astrocyte purity, highly reproducible isolation, and seamless compatibility with downstream molecular analyses.

The Bad

None observed in our experiments.

The Bottom Line

Anti-ACSA-2 MicroBeads provide a robust and reliable method for isolating adult mouse astrocytes for protein expression and metabolic studies.

Join the discussion