Proteintech
EEA1 Recombinant monoclonal antibody
81245-1-RR
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We are interested in understanding the function of a endososome-localized enzyme and use this anti-EEA1 antibody as endosomal marker to validate its subcellular localization. Using sucrose gradient centrifugation and western blotting, we are able to detect the enzyme in the EE1A-containing fraction.
Western Blot
HEK293T cells
1:1000 dilution overnight in the cold room
3% BSA in PBS
1:10000 goat anti-rabbit
1hr incubation at room temp
LICOR fluorescence
We are interested in understanding the function of a endososome-localized enzyme and use this anti-EEA1 antibody as an endosomal marker to validate its subcellular localization. Using sucrose gradient centrifugation and western blotting, we are able to detect the enzyme in the EEA1-containing fraction.
Specific and cost-effective
Nothing
Really good and cost-effect anti-EEA1 antibody that can be used as an endosomal marker in human cells.