Nuclear MED1 Staining by Immunofluorescence

Biochemistry
Stanford University
Postdoctoral Researcher

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Quality of Results

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Company:

Bethyl Laboratories

Product Name:

MED1 Recombinant Rabbit Monoclonal Antibody

Catalog Number:

A700-037

Clone Number:

BLR037F

I study signaling pathways and the transcriptional machinery that executes them. I use this MED1 antibody by immunofluorescence to visualize the Mediator complex subunit in nuclei and assess its distribution in response to signaling perturbations.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Fixed cultured cells

Primary Incubation

1:500

Blocking Agent

1%BSA, 5% Donkey Serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

AF-tagged secondary antibodies

Results Summary

This antibody produced clear nuclear staining with low cytoplasmic background, clearly resolving MED1 localization and enabling reliable comparison across treatment conditions.

Related Categories

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Summary

The Good

Also validated for Western blot

The Bad

none

The Bottom Line

Consistent antibody that gives crisp nuclear MED1 signal by IF

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