The Good
Simple one-step add-and-read;high sensitivity with wide linear range; low cytotoxicity
The Bad
Requires an incubation step and time must be optimized per cell line; endpoint reflects metabolic activity rather than a direct cell count
The Bottom Line
Results have been consistent plate to plate, and the kit is economical, which matters for large screens. Overall, this is a reliable, easy, and cost-effective CCK-8 kit, and I would recommend it to anyone doing routine viability or cytotoxicity work.