Proteintech
Lamin A/C Polyclonal antibody
10298-1-AP
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I frequently separate nuclear and cytoplasmic compartments to track where signaling proteins and transcription factors localize. I use this Lamin A/C antibody as a nuclear loading/fractionation marker by western blot to confirm clean subcellular separation.
Western Blot
Cell lysate
1:5000
5% milk in TBST
None
Chemiluminescence
Detected Lamin A/C as two clean, specific bands confined exclusively to the nuclear and total fractions with no cytoplasmic signal, confirming proper fractionation.
Exceptionally specific and sensitive
A gold-standard nuclear marker antibody for fractionation