Proteintech
Hamartin/TSC1 Polyclonal antibody
29906-1-AP
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I study gene signaling pathways centered on the TSC1/TSC2 complex and its negative regulation of mTORC1. I use this antibody to monitor TSC1 (Hamartin) levels by western blot when assessing how the complex controls growth and translation signaling.
Western Blot
Cell lysate
1:3000
5% milk in TBST
None
Chemiluminescence
Detected endogenous TSC1 as a specific that was lost in knockout controls, cleanly tracking the TSC1/TSC2 complex for mTOR pathway studies. Worked well in human cell lines.
N/A
KO-validated with specific signal
Some non-specific band in mouse cell line
A dependable TSC1/Hamartin antibody for mTOR signaling work