Proteintech
Tuberin/TSC2 Polyclonal antibody
24601-1-AP
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I study signaling pathways, with a focus on the TSC1/TSC2–mTORC1 axis that controls growth and nutrient sensing. I use this antibody to monitor TSC2 (Tuberin) levels by western blot IN TSC2 KO cells when probing how perturbations affect mTOR pathway activity.
Western Blot
Cell lysate
1:1000
5% milk in TBST
None
Chemiluminescence
Detected endogenous TSC2 as a single specific band that disappeared in sh/KO controls, cleanly confirming knockdown/knockout and enabling reliable pathway analysis.
N/A
KO-validated with clean, specific signal
none
knockout-validated TSC2 antibody that gives clean western blots for mTOR signaling studies