Clean, Sensitive mCherry Detection for Fusion Proteins

Biochemistry
Stanford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech

Product Name:

mCherry Polyclonal antibody

Catalog Number:

26765-1-AP

Clone Number:

Info:Supplier Page

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I study signaling pathways and use mCherry-fusion constructs as reporters to track protein expression and localization. I use this polyclonal antibody to detect my mCherry-tagged proteins by western blot when direct fluorescence isn't sensitive enough.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate

Primary Incubation

1:5000

Blocking Agent

5% milk in TBST

Secondary Incubation

None

Tertiary Incubation

None

Detection

Chemiluminescence

Results Summary

Detected mScarlet-tagged proteins as strong, specific bands with no background or nonspecific signal, cleanly distinguishing transfected from control lysates.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Very sensitive and clean

The Bad

None

The Bottom Line

A reliable, sensitive workhorse for detecting mCherry/RFP.mScarlet fusions by western blot

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