WedgeWell Gels with Easy High-Volume Loading

Stanford University
Biochemistry
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Questions/Comments

Company:

Invitrogen

Product Name:

Novex™ Tris-Glycine Mini Protein Gels, 4–12%, 1.0 mm, WedgeWell™ format

Catalog Number:

XP04125BOX

I study protein expression and signaling and run SDS-PAGE constantly ahead of western blotting. I use these 4–12% Novex WedgeWell gels to resolve a broad range of proteins and to load extra sample volume when my targets are low-abundance.

Experimental Design and Results Summary

Application

SDS-PAGE (denaturing protein separation for western blot)

Starting Material

Protein lysate in Laemmli/Tris-Glycine SDS sample buffer

Tips

The wedge-shaped wells take up to ~35 µL, so you can load 2× more without overflow into neighbors

Results Summary

These gels gave me well-resolved bands across 20–250 kDa with sharp separation and easy high-volume loading, giving clean transfers to membrane for blotting.

DOI or PMID #

N/A

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Summary

The Good

Wedge-shaped wells allow 2× sample loading with easy pipetting

The Bad

None

The Bottom Line

Convenient precast gel with standout high-volume loading

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