Detects pTAK1 at the Right MW

R&D
EydisBio Inc.
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Phospho-TAK1 (Thr187) Antibody

Catalog Number:

4536S

Clone Number:

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This detected pTAK1 but we did not see upregulation of pTAK1 after LPS. Samples were analyzed by westernblot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

human PBMC's

Primary Incubation

1:500

Blocking Agent

Normal Donkey Serum

Secondary Incubation

1:1000

Tertiary Incubation

None

Detection

HRP Westernblot Reader

Results Summary

This Ab detected pTAK1 at the right MW but we did not see any changes in pTAK1 after LPS stimulation. We tested at 5, 15 and 30 minutes post stimulation.

DOI or PMID #

N/A

Additional Notes

Image Gallery

Summary

The Good

May detect pTAK1

The Bad

Does not appear to be high affinity

The Bottom Line

May work but would need optimization and further validation.

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