Did Not Detect pTAK1 After LPS Stimulation

EydisBio Inc.
R&D
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Questions/Comments

Company:

LSBio

Product Name:

Mouse/Human/Rat Phospho-MAP3K7 / TAK1 (Thr187) (Cell-Based Phosphorylation ELISA) ELISA Kit

Catalog Number:

F1684

We used this ELISA to detect pTAK1 after LPS stimulation. We did not observe any pTAK1 expression in the positive control groups.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Tips

Did not detect pTAK1 well

Results Summary

This ELISA did not show robust pTAK1 in LPS stimulated human PBMC's. Maybe the timing can be optimized post stimulation but we expected some pTAK1 to show up.

DOI or PMID #

N/A

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Summary

The Good

Easy to use

The Bad

Did not detect pTAK1

The Bottom Line

This did not appear to work well for pTAK1.

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