Reliable, Sensitive, and Cost-effective ELISA Set for Quantifying Mouse IL-12 (p70)

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Company:

Biolegend

Product Name:

Elisa Max Deluxe Set Mouse IL12 p70

Catalog Number:

433604

The BioLegend ELISA MAX Deluxe Set (Cat# 433604) to quantify mouse IL-12 (p70) in mouse serum, and it performed excellently. The kit provides pre-optimized capture and detection antibodies, a standard, Avidin-HRP, substrate, and all required buffers, so the only additional item needed is the Nunc MaxiSorp plates.

Experimental Design and Results Summary

Application

Mouse Serum

Starting Material

Tips

Remember to run a standard curve each time

Results Summary

The standard curve was highly linear and reproducible across the working range, with low background and good well-to-well consistency. Sensitivity was more than sufficient to detect physiologically relevant IL-12 (p70) levels in my samples.

DOI or PMID #

N/A

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Summary

The Good

Sensitive, reproducible standard curve; complete reagent set with anti-interference buffers; cost-effective per plate; clear protocol and responsive technical support

The Bad

Plates not included and require overnight coating, so it needs a bit more hands-on time than a pre-coated kit.

The Bottom Line

The protocol is straightforward and well documented. Overall, this is a dependable, sensitive, and budget-friendly ELISA set that I would recommend to anyone measuring mouse IL-12 (p70).

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