Excellent Fluorescent Dye for Labeling Gram-Positive Bacteria Like Enterococcus

July 11, 2026

BlueskyReddit
The Scripps Research Institute
Immunology Microbiology
Postdoctoral Researcher

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Company:

Tocris Bioscience

Product Name:

sCy5DL-amide

Catalog Number:

7835

My research focuses on understanding the cell envelope biology of Enterococcus using super-resolution microscopy. I evaluated the labeling efficiency of sCy5DL-amide for fluorescently labeling Enterococcus cells and assessing its suitability for high-resolution imaging.

Experimental Design and Results Summary

Application

Fluorescent labeling of Gram-positive bacteria, including Enterococcus for super-resolution microscopy

Starting Material

Exponentially growing bacterial cells

Tips

For optimal labeling, I stained exponentially growing Enterococcus cells with 0.5 mM sCy5DL-amide for 15 minutes at 37°C in a shaking incubator.

Results Summary

sCy5DL-amide provided excellent labeling of Enterococcus cells with strong, bright fluorescence and a high signal-to-background ratio. The labeling was consistent across the bacterial population, making it well suited for super-resolution microscopy. From the image, a characteristic tri-septal labeling pattern was clearly visible, indicating active cell wall remodeling at the septa and equatorial regions during cell growth and division. This distinct labeling pattern enabled clear visualization of cell wall dynamics with high spatial resolution.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Bright and uniform labeling

The Bad

None

The Bottom Line

sCy5DL-amide is an excellent dye for labeling Gram-positive bacteria such as Enterococcus. It provides bright, consistent labeling and is highly suitable for super-resolution microscopy applications.

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