Accurate, dsDNA-specific Quantitation

Stanford University
Biochemistry
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Questions/Comments

Company:

Invitrogen

Product Name:

Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kit

Catalog Number:

Q33230

I use this kit to accurately measure dsDNA concentration on a Qubit fluorometer, since specific quantitation matters more than the crude readings a nanodrop gives.

Experimental Design and Results Summary

Application

Fluorometric dsDNA quantitation

Starting Material

Purified dsDNA

Tips

The 1X ready-to-use working solution is a big upgrade, no more mixing dye and buffer fresh each time.

Results Summary

Delivered accurate, reproducible dsDNA concentrations down to the picogram range for reliable input for my NGS runs.

DOI or PMID #

N/A

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Summary

The Good

Highly dsDNA-selective

The Bad

None

The Bottom Line

The go-to for accurate, sensitive dsDNA quantitation and far more reliable than absorbance methods for sensitive downstream applications

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