Fast Genotyping Straight from Mouse Tails

Stanford University
Biochemistry
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review
Questions/Comments

Company:

Sigma-aldrich

Product Name:

Extract-N-Amp™ Tissue PCR Kit

Catalog Number:

XNAT2-1KT

I use this kit to extract genomic DNA from tail biopsies and amplify targets directly, without column purification or overnight digestion.

Experimental Design and Results Summary

Application

Direct-PCR genotyping of mouse tail biopsies

Starting Material

Mouse tail snips

Tips

Extract stays stable at 4°C for months, so you can batch extractions ahead of PCR.

Results Summary

Extracted PCR-ready genomic DNA in ~15 minutes and got specific, reproducible genotyping bands directly from crude tail lysate

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Extremely fast and simple

The Bad

Crude extract carries PCR inhibitors that can require dilution/optimization

The Bottom Line

A convenient, dependable kit that turns tail snips into genotyping results in under an hour

Join the discussion