Abcam
Goat anti-Mouse Secondary Antibody
ab97023
Info:Supplier Page View Product Specs
Browse Similar Products: Secondary Antibodies
My research focuses on understanding how cell wall remodeling affects cell growth and response to stress. I used this secondary antibody for immunoblot experiments to detect target proteins after transferring samples to membranes. It consistently produced clear and easy-to-read bands.
Western Blot
Enterococcus faecium
Primary antibody diluted according to the manufacturer’s recommendation (1:2,000). Incubate overnight at 4°C
5% non-fat dry milk powder in TBST
Goat Anti-Mouse IgG H&L (HRP), 1:10,000 dilution in blocking buffer, 1 hour at room temperature.
NA
Enhanced Chemiluminescence (ECL) using an HRP substrate; image with a chemiluminescence imaging system.
I used this secondary antibody to detect a HaloTag-fused protein by western blot. It produced strong, clean bands with very low background, allowing me to reliably monitor the stability of the protein fusion under different experimental conditions. Confirming stable expression by western blot gave me confidence for downstream fluorescence microscopy studies, where the HaloTag fusion was used to examine the cellular localization of the protein. The HRP-conjugated secondary antibody consistently provided high sensitivity and reproducible results throughout the study.
N/A
Very consistent performance
none
It has a great signal and delivers clean and reproducible western blot results.