Robust Presynaptic Puncta Labeling for Quantitative Synapse Analysis

July 07, 2026

BlueskyReddit
SUNY UB
Research Scientist

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Company:

Thermo Fisher Scientific

Product Name:

VGLUT1 Antibody

Catalog Number:

48-2400

Clone Number:

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We used this antibody to visualize glutamatergic presynaptic terminals in the mouse prefrontal cortex. The antibody produced robust punctate staining and was compatible with high-resolution confocal imaging and 3D quantitative analysis. Combined with PSD95 immunostaining, it allowed us to evaluate closely associated pre- and postsynaptic excitatory markers within individual astrocyte territories.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

40-μm free-floating mouse prefrontal cortex brain sections

Primary Incubation

1:1000 overnight at 4°C

Blocking Agent

3% normal goat serum + 0.3% Triton X-100 in PBS, 1 hour at room temperature

Secondary Incubation

Corresponding Alexa Fluor–conjugated secondary antibody, 1:1000, 2 hours at room temperature

Tertiary Incubation

NA

Detection

Confocal microscopy (Leica SP8) and quantitative 3D analysis using Imaris

Results Summary

VGLUT1-positive terminals were clearly visualized as discrete puncta and could be reliably detected during 3D image analysis. The staining was reproducible between samples and allowed quantitative assessment of excitatory synaptic puncta density and distance from reconstructed astrocyte surfaces.

DOI or PMID #

DOI: 10.1126/sciadv.adt2527

Additional Notes

Image Gallery

Summary

The Good

Strong punctate labeling, reproducible staining, and excellent compatibility with multiplex confocal imaging and 3D analysis.

The Bad

Dense punctate labeling requires careful and consistent thresholding during quantitative image analysis.

The Bottom Line

A dependable antibody for visualization and quantitative analysis of glutamatergic presynaptic terminals in mouse brain tissue.

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