Specific for Dengue1-4

R&D
EydisBio Inc.
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Thermo Fisher

Product Name:

DENGUE VIRUS TYPE 1-4 ANTIBODY

Catalog Number:

MA127093

Clone Number:

This antibody worked well to detect viral infection in vero cells in a 96 well format. Cells were infected with Dengue serotypes for 3 days and viral infection detected by ICC.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Vero Cells

Primary Incubation

1:1000

Blocking Agent

Normal Donkey Serum

Secondary Incubation

1:2000

Tertiary Incubation

None

Detection

Plate Reader

Results Summary

We used this Ab to detect viral infection in vero cells. Cells were treated in a 96 well plate for 3 days and infection determined by antibody staining. Secondary Ab was used to visualize at 488nm.

DOI or PMID #

N/A

Additional Notes

Image Gallery

Summary

The Good

Detects viral infection in infected samples

The Bad

Needs some optimization and overnight incubation

The Bottom Line

This worked well and was specific to dengue 1-4. As it works against all 4 serotypes it allows for use across multiple infections.

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