Excellent Fixation with Consistent Cell Preservation

June 25, 2026

BlueskyReddit
MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Fixative Solution, 4% Formaldehyde

Catalog Number:

FB002

A high-quality ready-to-use fixative that consistently preserves cellular morphology and marker expression. It simplifies workflows while providing reproducible fixation across experiments.

Experimental Design and Results Summary

Application

Fixation prior to flow cytometry, mass cytometry (CyTOF), immunostaining, imaging, and other cell-based assays.

Starting Material

Cultured cells, PBMCs, splenocytes, primary immune cells, dissociated tissues, and cell suspensions intended for downstream analysis.

Tips

Keep it refrigerated, avoiding multiple in/out. Use freshly aliquoted solution and avoid prolonged exposure to room temperature.

Results Summary

Provides consistent fixation with excellent preservation of cellular structure and staining quality. Samples remain stable and compatible with downstream assays.

DOI or PMID #

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Summary

The Good

Ready-to-use formulation with no dilution required. Excellent preservation of cellular morphology. Consistent performance across experiments.

The Bad

Requires careful handling due to formaldehyde content. Limited shelf life once opened. More expensive than preparing fresh fixative solutions.

The Bottom Line

An excellent fixative that delivers dependable results with minimal preparation.

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