Maxpar™ Delivers Consistent CyTOF Staining

MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Fluidigm Corp

Product Name:

Maxpar™ Cell Staining Buffer

Catalog Number:

201068

A dependable buffer for mass cytometry workflows that helps reduce nonspecific staining and maintain cell integrity during antibody labeling. It consistently delivers clean staining and reproducible results, although the cost can add up for high-throughput studies.

Experimental Design and Results Summary

Application

Cell staining and washing during mass cytometry (CyTOF) workflows, antibody labeling, sample preparation, and minimizing nonspecific binding.

Starting Material

Cell suspensions from PBMCs, whole blood, cultured cell lines, dissociated tissues, and tumor samples for antibody staining, washing, and preparation in mass cytometry (CyTOF) workflows.

Tips

Keep the buffer refrigerated and use consistent staining conditions across samples to maximize reproducibility.

Results Summary

Provides clean staining patterns with low background and good cell recovery. Cells remain stable throughout staining procedures, and antibody panels perform consistently across experiments. Particularly useful for complex multi-parameter CyTOF assays where reproducibility is essential.

DOI or PMID #

N/A

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Summary

The Good

Reduces nonspecific antibody binding and background signal. Improves staining consistency across experiments. Ready-to-use formulation saves preparation time. Supports reproducible CyTOF data generation.

The Bad

More expensive than preparing staining buffer in-house. Requires refrigerated storage and proper handling. Limited utility outside mass cytometry workflows.

The Bottom Line

Excellent performance for CyTOF staining, but the premium price prevents it from being a perfect score.

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