Strong in Human, Weak in Mouse: Optimizing Antibody Performance for Reliable Tissue Imaging

June 23, 2026

BlueskyReddit
Medicine
Cedars Sinai Medical Centre
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Novus Biologicals

Product Name:

CD45 Antibody (EM-05) [Alexa Fluor® 594]

Catalog Number:

NBP1-44763AF594

Clone Number:

N/A

I used this antibody for an immunofluorescence assay in mouse and human tissue, and it worked really well for human tissue.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse, human

Primary Incubation

1:40 in 5% BSA

Blocking Agent

5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fluorescence microscopy

Results Summary

It did work really nice with human tissue but did not work that well with mouse tissue. Antibody optimization is required for mouse tissue. Yellow color represents CD45 staining in images provided below.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well with Human tissue.

The Bad

The antibody needs a higher concentration to get good image with mouse tissue.

The Bottom Line

I recommend using this product for human tissue samples but with mouse tissue it need concentration optimization.

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