Enhancing Cytokeratin 8/18 Detection: Requirement for Higher Antibody Concentration in Novus Biologicals Assay

June 23, 2026

BlueskyReddit
Medicine
Cedars Sinai Medical Centre
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Cytokeratin 8/18 Antibody (K8.8 + DC10) [Alexa Fluor® 647]

Catalog Number:

NBP2-34655AF647

Clone Number:

N/A

I used the antibody for GeoMx and it worked really nice. However the mouse application was not mentioned on suppliers page but it did work well with my mouse liver tissue.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse

Primary Incubation

1:100 in 5% BSA

Blocking Agent

5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fluorescence microscopy

Results Summary

Antibody is really good and help me visualize adjacent liver in cancer metastatic zone. Although the mouse application was not mentioned by supplier but it did work really well.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well with mouse tissue.

The Bad

Need higher concentration to get good image.

The Bottom Line

It is very good antibody for detecting liver epithelial cell. I would recommend using for both mouse and human tissue.

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